Description
Intended Uses: The Invitron Intact Proinsulin ELISA kit is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.
Principle of the Assay: The Invitron Intact Proinsulin Assay is a two-site enzyme-linked immunosorbent assay (ELISA), employing a specific solid phase antibody immobilised on microtitre wells and a soluble antibody labelled with biotin. The sample is incubated in the microtitre well together with a buffer and, after a wash step, the biotin labelled antibody solution is added. After a second incubation and wash step, HRP labelled streptavidin is added. A third incubation and wash is followed by the addition of substrate solution. Following colour development "stop reagent" is added and the colour intensity measured in a 96-well microplate reader